Rajeshkumar et al. World Journal of Pharmacy and Pharmaceutical Sciences. ANTICANCER ACTIVITY OF ANDROGRAPHIS PANICULATA. showing anticancer activity on HEp2 – Human larynx carcinoma cell lines in-vitro KEYWORDS: Andrographis paniculata,Herbal medicine. A refined extract of A. paniculata, also significantly reduces activities of lipid .. The anticancer activity was observed due to the presence of andrographolide.

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From natural products to drug discovery. All chemicals used were of analytical grade.

BioMed Research International

But many cancer patients seek alternative and complementary methods of treatment because of the serious side effects of chemotherapy and radiation therapy. To be clearer, it seems that the accessions with high contents of the anticancer phytochemicals under normal condition would deal more successfully with salinity, as well.

The Graphpad Prism software version 5 was used for drawing the graphs. The following drugs and chemicals were used: Hepatoprotective and antioxidant property of Andrographis paniculata Nees in BHC induced liver damage in lf.

These compounds are produced mainly from aerial organs of the plant, panculata leaves. Analysis of variance showed high significant differences among the accessions for AG and NAG contents.

Subscribe to Table of Contents Alerts. The solvent extract was concentrated under reduced pressure using a rotary evaporator. The concentration of the AG, the most important cytotoxic principle in A. This event complies with the hypothesis that the A.

However, to prove their efficacy for clinical utilization more scientific data are needed.

After 30 days of culturing almost in 70 days oldthe plants were placed in different salinity levels. These medicinal plant s possess good immunomodulatory and antioxidant properties, leading to anticancer activities.

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The day seedlings were transferred from jiffy media into the pot with sand medium. Variance analysis of salinity effects on morphological traits, proline, and the three main phytochemicals in A. Consequently, the results suggested that increasing the biosynthesis of proline in the plant could protect the cellular structures from oxidative damage and osmotic stress. Gour Central University, Sagar M.

Salinity causes the adverse effects in all physiological processes of plants. Cancer is the uncontrolled growth and division of cells and it leads to a mass pxniculata cells known as tumor. The effects of salinity levels on studied traits among the four accessions of A.

For example, the level of metabolites was increased in the leaves of salt-treated plants such as Arabidopsis thaliana [ 4 ], Oryza sativa [ 5 ], Hordeum vulgareDatura inoxiaGlycine maxTriticum aestivum [ 5 ], and Catharanthus roseus [ 6 ].

Apart from this, alkaloids were present only in the extract of Andrographis paniculata while, saponins were andrographls only in Qnticancer marianum extract Table 1. Andrographis paniculata extract was found most effective against Ovcar cell line Under the low level of salinity, the salt ions inhibited the biosynthesis of phytochemicals as indicated by the decrease in their amounts in both tolerant and sensitive accessions.

The results obtained in the study indicate that A. The authors would like to thank Mr. Anddrographis cultures were passage weekly and the culture medium was changed twice a week. Enhancement of phenolic and flavonoid compounds in onion plant under salinity stress has been reported to improve the deleterious effect of salinity stress [ 34 ].


The human tumor cytotoxicity was determined by the protocols established by NCI Monks et al.

Solvents AR and chromatography grade for isolation and purification of the compounds were used as supplied by Fisher Scientific Leicestershire, UK. The results showed that proline, AG, and NAG contents were positively correlated to the substrate concentration of NaClwhile the total crude extract amount was negatively correlated to salinity levels Figure 2. View at Google Scholar L.

List of the 4 accessions of Andrographis paniculata collected from Peninsular Malaysia. Phytochemicals, such as flavonols and flavonoids were investigated to determine chemoprevention activity against cancer Conese and Blasi, Flavonoids isolated from Plantago species were able to strongly inhibit the proliferation of human cancer cell lines Galvez et al.

Analysis of the Anticancer Phytochemicals in Andrographis paniculata Nees. under Salinity Stress

After inducing a period of day salinity stress and before flowering, all plants were harvested and the data on morphological traits, proline content and the three anticancer phytochemicals, including andrographolide AGneoandrographolide NAGand deoxy,didehydroandrographolide DDAGwere measured.

In spite of the mentioned trends, the differences between those two accessions were not statistically significant [ 36 ]. In other words, the increase of these phytochemicals under salinity condition would be partially compensated by a decline in total biomass.